Troponin-I Test Kit

Purpose and Mechanism

The troponin-I test kit is used to measure the abnormal levels of cardiac troponin in human blood serum/plasma, thereby helping to identify the risk of myocardial infarction. Troponin I is a biomarker and is released shortly after the onset of acute myocardial infarction. The level of troponin-I should be below an average level in a healthy person; therefore, diagnosis of its elevated levels in a person with suspected AMI is necessary. The test kit works on the principle of chromatographic immunoassay. When the sample is added to the sample pad, it moves across and reacts with the specific antibodies, forming a distinct line.

User Guide

• Bring the kit and specimen to normal room temperature well before using them.
• Remove the card from the package and keep it on a flat surface.
• Using a dropper, fill the sample well with 2-3 drops (about 90 μL) of the specimen.
• Keep the card for 10-15 minutes and then interpret the results.

Interpretation

• Negative: When only a single line appears on the control region of the card, it indicates a negative result for high levels of troponin-I.
• Positive: When two lines appear on the card, one for the test region and one for the control region, it indicates a positive for the high levels of troponin-I.
• Invalid: When no lines are present on the card, it indicates that either the reagent has deteriorated or the test was performed incorrectly.