Syphilis Ab Test Kit

Purpose and Mechanism

Syphilis Ag is a rapid test card used to detect syphilis antigen in human blood serum/plasma. Syphilis is a sexually transmitted infection that can cause severe organ damage if left untreated. The card utilizes a chromatographic technique to detect IgA, IgM, and IgG generated against Treponema pallidum antigens (17 kDa, 15 kDa, and 47 kDa). In the card, these antigens are immobilized on the test region. When the specimen passes through the strip, the antibodies attach to the antigens, forming a line on the test region. For control, goat anti-mouse IgG antibodies are immobilized at the end of the strip.

User Guide

• Remove the card from the pouch and keep it on a flat surface.
• Bring the card and specimen both to room temperature.
• Using a dropper, place 2 to 4 drops of the specimen into the sample well.
• Let the card sit for 15-20 minutes and then interpret the results.

Interpretation

• Negative: Only one line appears on the control region (C); then the result is negative for syphilis.
• Positive: With a line on the control region (C), there is also a line on the test region (T). This indicates a positive result for syphilis.
• Invalid: A total absence of lines on the card indicates either that the procedure was conducted incorrectly or the reagent has deteriorated.