Prostate-Specific Antigen Test Kit

Purpose and Mechanism

The PSA test kit is a diagnostic tool to detect prostate-specific antigens in whole blood/serum/plasma. This screening can help identify prostate cancer and other related cancers. The PSA kit is based on the effective principle of a lateral flow immunochromatographic assay in a sandwich format. It contains a conjugate pad, which is coated with anti-PSA antibodies labeled with a visible tag such as colloidal gold. When the buffer is applied, prostate-specific antigens bind to these labeled antibodies, creating a distinct line.

User Guide

• Bring the specimen and test kit to room temperature well in advance of the procedure.
• Using the dropper, fill approximately 10-20 µL of the specimen into the sample well.
• To initiate the flow, add 2-3 drops of the assay buffer to the buffer well.
• Wait for 15-20 minutes before interpreting the result.

Interpretation

• Negative: When the card contains only one line in the control region, it indicates negative results for the PSA.
• Positive: When two lines are present on the card, it indicates the presence of prostate-specific antigens in the sample.
• Invalid: When there are no lines on the card, it indicates that the reagent has been compromised or that the procedure was not conducted properly.