Malaria Pf/Pv Test Kit

Purpose and Mechanism

The malaria Pf/Pv card is a fast diagnostic device for detecting P. falciparum and P. vivax, the two primary parasites that cause malaria in humans. In areas where infections are mixed, this test can help distinguish between the two. Malaria is a fatal, parasitic disease that can cause cerebral fever. The rapid test kit works on the principle of immunochromatography. The membrane of the card is coated with monoclonal anti-HRP II antibody (works as a test line for PF) and monoclonal anti-Pan pLDH antibody (works as a test line for PV). For control, goat anti-mouse IgG antibodies are immobilized at the end of the membrane. When the specimen mixed with buffer flows through the membrane, it binds to the colloidal gold conjugated to the specific antibodies. This complex becomes immobilized on the test line, producing a distinct line.

User Guide

• Remove the card from the pouch and place it on a flat surface.
• Fill 5 µl of whole blood into the sample well of the card.
• Add exactly 3 drops (90 µl) of assay buffer to the buffer well.
• After exactly 5 minutes of adding specimen and buffer, add one more drop of assay buffer for better results.
• Let the card sit for 20 minutes and then interpret the results.

Interpretation

• Negative: A single line on the control region indicates a negative result for Malaria Pf/Pv.
• Positive Pf: If there are two lines, one on the Pf test region and one on the control region, then the result is positive for Malaria Pf.
• Positive Pv: If there are two lines, one on the Pv test region and one on the control region, then the result is positive for Malaria Pv.
• Mixed Infection: Along with a line on the control region, if there are lines on both Pf and Pv regions, then the result is positive for both types, also known as a mixed infection.
• Invalid: If there is no line on the card, then it means either the procedure was conducted incorrectly or the reagent has deteriorated.