Leptospira IgG/IgM Test Kit

Purpose and Mechanism

The Leptospira IgG/IgM rapid test kits are used for the rapid detection of immunoglobulin G and immunoglobulin M generated against Leptospira (a species of bacteria) in human blood. It can cause severe leptospirosis, resulting in fever, liver infection, kidney failure, etc. Therefore, it is essential to diagnose and treat it promptly. A lateral flow immunochromatographic assay is employed in the Leptospira IgG/IgM rapid test. It includes an IgG-gold conjugate along with a conjugate pad containing Leptospira interrogans-specific antigen and colloidal gold. It has a nitrocellulose membrane with two regions: a test region and a control region. The test line “G” is coated with an anti-human IgG, and the test line “M” is coated with anti-IgM. For control, the membrane at last contains goat anti-mouse IgG. When the specimen is added to the sample well, the IgG and IgM antibodies react and form complexes.

User Guide

• Bring the specimen and the card to room temperature.
• Remove the card from the pouch and place it on a flat surface.
• Add 5 µl of specimen to the sample well of the card.
• Add 2-3 drops (100 µl) of assay buffer into the sample well.
• Let the card sit for 15 minutes and then interpret the results.

Interpretation

• Negative: Appearance of a single line on the control region indicates a negative result for the Typhoid IgG/IgM antibody.
• Positive IgG: If there are two lines, one on the IgG test region and one on the control region, then the result is positive for the IgG antibody against L. interrogans.
• Positive IgM: If there are two lines, one on the IgM test region and one on the control region, then the result is positive for the IgM antibody against L. interrogans.
• IgG and IgM Positive: If there are three lines on the card, one for IgG, one for IgM, and one for the control region, then the sample is positive for both IgG and IgM.
• Invalid: If the card shows no line, the reagent may have degraded, or an error occurred during execution.