HIV 1/2 Triline Ab Test Kit

Purpose and Mechanism

The HIV 1/2 test is a solid-phase immunochromatography assay to detect antibodies against HIV-1 and HIV-2 in whole blood/serum/plasma. Both types of HIV are identical in their external shape, cell tropism, host interaction, and genetic makeup. Our rapid HIV 1/2 triline card utilizes a lateral flow chromatographic test. It contains a nitrocellulose membrane strip with colloidal gold combined with recombinant antigens (Au-Ag), corresponding to HIV-1 with glycoproteins 120 and 41 and HIV-2 with glycoprotein 36. The antigens are bound at test regions 1 and 2 (T1, T2), and goat anti-mouse IgG is bound at the control region. If there is a line in either T1 or T2, the test is positive. The antibodies present in the serum are destined to bind the gold-antigen complex.

User Guide

• Remove the test from the pouch and keep it on a flat surface.
• Bring both the card and specimen to room temperature.
• Using a dropper, add 10 µl of the specimen to the sample well.
• Add about 60 µL of assay buffer to the sample well.
• Keep the test card for 15-20 minutes, and then interpret the result.

Interpretation

• HIV-1 Positive: A line appearing on the test region T1 and the control region indicates positive for HIV-1.
• HIV-2 Positive: A line appearing on the test region T2 and the control region indicates positive for HIV-2.
• Both HIV 1-2 Positive: When a line appears in both the T1 and T2 regions, along with a line in the control region, it indicates a positive result for both.
• Negative: The appearance of one line on the control region and no other line on the T1 and T2 regions indicates a negative result for both HIV-1 and HIV-2.
• Invalid: A total absence of lines on all three regions indicates an error in the procedure or the test reagent deterioration.