HBsAg Test Kit

Purpose and Mechanism

The HBsAg card is a rapid test kit used for detecting the hepatitis B surface antigen (HBsAg) in human blood serum/plasma. Hepatitis B is a sexually transmitted or bloodborne disease. It can cause acute or chronic infection and liver cirrhosis. The hepatitis antigen consists of lipid, carbohydrate, and protein elements, which work as markers for identification. Our rapid test kit works on the principle of a sandwich immunoassay. It contains a nitrocellulose strip with immobilized monoclonal antibodies conjugated with colloidal gold. To control the flow, the end of the strip includes goat anti-mouse IgG. When the sample is introduced to the card, it flows through the pad, and if it contains antigens, it binds to the gold-antibody complex.

User Guide

• Remove the card from the pouch and place it on a flat surface.
• Bring the specimen and card to room temperature.
• Use a dropper to add 2-3 drops of the specimen into the sample well.
• Keep the card aside for 15-20 minutes and then interpret the results.

Interpretation

• Negative: A single line on the control region indicates a negative result for HBsAg.
• Positive: If there are two lines, one in the test region and one in the control region, then the result is positive for HBsAg.
• Invalid: If there is no line on the card, then it indicates an error in the procedure or deterioration of the reagent.