Dengue Antibody IgG/IgM - NS1 Test Kit

Purpose and Mechanism

The dengue antibody IgG/IgM kit is used for the rapid detection of immunoglobulin G and immunoglobulin M in all types of dengue viruses, utilizing human blood serum/plasma. The test device contains three pre-coated lines: “G” for IgG, “M” for IgM, and “C” for control lines. A dengue-specific antigen complexed with a gold conjugate is placed in the conjugate pad with anti-human IgG and IgM fixed on the membrane. When the specimen is added to the sample well, the dengue antigen reacts with the antibodies and forms complexes. Anti-human antibodies capture these complexes to make a line. For control, the membrane at last contains goat anti-mouse IgG.

The dengue antibody NS1 test is used for the rapid detection of dengue antigen NS1 in human blood serum/plasma. NS1 is a glycoprotein found in dengue-infected persons during the early days of infection. The card contains a membrane pre-coated with an anti-dengue NS1 monoclonal antibody in the test region and goat anti-mouse IgG in the control region. As the specimen flows, if it contains dengue NS1 antigens, it will form a complex called an antibody-antigen-gold conjugate, which moves laterally on the membrane. This will create a distinct line on the card in addition to a control line.

User Guide For Dengue Antibody IgG/IgM

• Bring the kit and specimen to room temperature.
• Remove the card from the pouch and place it on a flat surface.
• Add 10 µl of the specimen to the sample well of the card.
• Add 2-3 drops (75-90 µl) of assay buffer into the buffer well.
• Let the card sit for 20 minutes and then interpret the results.

User Guide For Dengue Antigen NS1

• Let the card and specimen come to room temperature.
• Remove the card from the pouch and place it on a flat surface.
• Add 50-75 µL of the specimen to the sample well on the card.
• Let the card sit for 20 minutes and then interpret the results.

Interpretation for IgG/IgM Test Kit

• Negative: Appearance of a single line on the control region indicates a negative result for the dengue IgG/IgM antibody.
• Positive IgG: If there are two lines, one on the IgG test region and one on the control region, then the result is positive for dengue IgG antibody.
• Positive IgM: If there are two lines, one on the IgM test region and one on the control region, then the result is positive for dengue IgM antibody.
• IgG and IgM Positive: If there are three lines on the card, one for IgG, one for IgM, and one for the control region, then the sample is positive for both IgG and IgM. It indicates late primary or early secondary infection.
• Invalid: If the card has no line, the reagent has degraded, or there was a mistake in the execution.

Interpretation For the NS1 Test Kit

• Negative: Appearance of a single line on the control region indicates a negative result for the dengue antigen NS1.
• Positive NS1: If there are two lines, one in the test region and one in the control region, then the result is positive for dengue antigen.
• Invalid: If the card has no line, the reagent has either degraded or there was a mistake in the execution during the test.