Dengue Antibody IgG/IgM Test Kit

Purpose and Mechanism

Dengue antibody IgG/IgM test kits are used for the rapid detection of immunoglobulin G and immunoglobulin M in all types of dengue viruses using human blood serum/plasma. Dengue is a prevalent infection in tropical and subtropical regions worldwide. It is a fatal disease due to its morbidity and mortality rates. There are four distinct types of dengue viruses, namely dengue viruses 1, 2, 3, and 4. The test device contains three pre-coated lines: “G” for IgG, “M” for IgM, and “C” for control lines. A dengue-specific antigen complexed with gold conjugate is placed in the conjugate pad with anti-human IgG and IgM fixed on the membrane. For control, the membrane at last contains goat anti-mouse IgG. When the specimen is added to the sample well, the dengue antigen reacts with the antibodies and forms complexes. Anti-human antibodies capture these complexes to make a line.

User Guide

• Remove the card from the pouch and place it on a flat surface.
• Add 10 µl of the specimen to the sample well of the card.
• Add 2-3 drops (75-90 µl) of assay buffer into the sample well.
• Let the card sit for 20 minutes and then interpret the results.

Interpretation

• Negative: Appearance of a single line on the control region indicates a negative result for the dengue IgG/IgM antibody.
• Positive IgG: If there are two lines, one on the IgG test region and one on the control region, then the result is positive for dengue IgG antibody.
• Positive IgM: If there are two lines, one on the IgM test region and one on the control region, then the result is positive for dengue IgM antibody.
• IgG and IgM Positive: If there are three lines on the card, one for IgG, one for IgM, and one for the control region, then the sample is positive for both IgG and IgM. It indicates late primary or early secondary infection.
• Invalid: If the card has no line, the reagent has either degraded or there was a mistake in the execution during the test.